| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhu, Stuart (14)
|
MDEST |
2:32.94 |
2:33.48 |
0.54 |
| 2 |
Stein, Jonah C (14)
|
MDRAC |
3:07.51 |
2:57.98 |
-9.53 |
| 3 |
Zhu, Roger Y (13)
|
MDEST |
3:06.73 |
3:05.05 |
-1.68 |
| 4 |
Davis, Gailen V (14)
|
MDEST |
3:02.87 |
3:05.35 |
2.48 |
| 5 |
Vahlsing, Joshua J (14)
|
MDRAC |
3:30.69 |
3:07.74 |
-22.95 |
| 6 |
O'Donnell, Matthew B (13)
|
MDEST |
3:12.88 |
3:09.57 |
-3.31 |
| 7 |
Almodovar, Jayden J (13)
|
MALAC |
3:10.60 |
3:10.26 |
-0.34 |
| 8 |
Kong, Matthew (13)
|
MDEST |
3:14.05 |
3:11.10 |
-2.95 |
| 9 |
Maharjan, Jenish (14)
|
MDEST |
3:13.86 |
3:17.15 |
3.29 |
| 10 |
Damico, Nicholas T (13)
|
MDRAC |
3:32.71 |
3:17.56 |
-15.15 |
| 11 |
Keiner, Ethan J (13)
|
MDEST |
3:48.79 |
3:19.08 |
-29.71 |
| 12 |
Parhi, Tanush (14)
|
MDEST |
3:19.83 |
3:19.63 |
-0.20 |
| 13 |
Pell, Vincent H (13)
|
MDEST |
4:08.94 |
3:20.63 |
-48.31 |
| 14 |
Zhang, Ryan D (13)
|
MDEST |
3:21.58 |
3:25.50 |
3.92 |
| 15 |
Speace, Hayden H (13)
|
MDEST |
3:29.71 |
3:26.33 |
-3.38 |
| 16 |
von Wachter, Noah G (13)
|
MDRAC |
3:36.83 |
3:27.34 |
-9.49 |
| 17 |
Williams, Benjamin A (13)
|
MDRAC |
3:39.99 |
3:31.33 |
-8.66 |
| 18 |
Qian, Simon C (13)
|
MDEST |
3:42.79 |
3:41.41 |
-1.38 |
| 19 |
Sloan, Logan M (13)
|
MDEST |
3:40.43 |
3:44.29 |
3.86 |